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Cell surface protein engineering for high-performance whole-cell catalysts

Hajime Nakatani,Katsutoshi Hori

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1,   Pages 46-57 doi: 10.1007/s11705-017-1609-3

Abstract: Cell surface protein engineering facilitated by accumulation of information on genome and protein structure, and is important for the development of high-performance whole-cell catalysts., on the cell surface using a carrier protein.discuss advanced methods for displaying multiple enzymes and a new method for the immobilization of whole-cellcatalysts using adhesive surface proteins.

Keywords： cell surface engineering     surface display     whole-cell catalysts     bioprocess    

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Efficient acetoin production from pyruvate by engineered whole-cell biocatalysis

Frontiers of Chemical Science and Engineering 2023, Volume 17, Issue 4,   Pages 425-436 doi: 10.1007/s11705-022-2229-0

Abstract: Moreover, the reusability of the cell catalysis was also tested, and the result illustrated that thewhole-cell catalysis obtained 433.3, 440.2, 379.0, 442.8 and 339.4 mmol·L–1 (38.2, 38.8, 33.4This work therefore provided an efficient H. bluephagenesis whole-cell catalysis with a broad

Keywords： -acetolactate synthetase     α-acetolactate decarboxylase     Halomonas bluephagenesis     whole-cell    

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Efficient production of D-1,2,4-butanetriol from D-xylose by engineered

Shewei Hu, Qian Gao, Xin Wang, Jianming Yang, Nana Xu, Kequan Chen, Sheng Xu, Pingkai Ouyang

Frontiers of Chemical Science and Engineering 2018, Volume 12, Issue 4,   Pages 772-779 doi: 10.1007/s11705-018-1731-x

Abstract: We have developed a whole-cell bioconversion system for the production of D-1,2,4-butanetriol (BT) fromA plasmid pETduet- T7- T7- was constructed and transformed to BL21(DE3) to obtain the whole cellsmetal ions, and culture conditions (pH, temperature, IPTG) were identified, and their effects on the whole-cellTo obtain the highest whole-cell activity, the optimal cultivation parameters are: 15 g·L yeast extractWith the optimized whole cells of BL21- , 60 g·L of xylose was converted to 28g·L BT with a molar

Keywords： D-1     2     4-butanetriol     whole-cell bioconversion     carbon source     nitrogen sources     metal ions     culture conditions    

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Organizational evolution of project management teams over the whole lifecycle of megaprojects: Case study

Frontiers of Engineering Management   Pages 439-454 doi: 10.1007/s42524-022-0211-7

Abstract: Project management teams are critical in the implementation of megaprojects, but their evolution throughout the project lifecycle has not been clearly explained. This paper explores the organizational evolution of megaproject management teams through a longitudinal retrospective case study of the Hong Kong–Zhuhai–Macao Bridge (HZMB) project. The organizational evolution is examined in terms of management objectives, management content, and organizational structure. The organizational evolution of the HZMB project management team exhibits stage differentiation with the coexistence of turbulence and stability. Changes in the external environment are the driving force for organizational evolution, whereas a flexible organizational strategy is critical in promoting this evolution. Basing on the HZMB case study, this paper summarizes six critical measures that facilitate the organizational evolution of megaproject management teams. Our findings add value to megaproject management theory and provide a better understanding of the dynamics and complexity of megaproject organizational management.

Keywords： megaproject     organizational evolution     whole lifecycle management     project management team    

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TCGA whole-transcriptome sequencing data reveals significantly dysregulated genes and signaling pathways

Daniel Wai-Hung Ho,Alan Ka-Lun Kai,Irene Oi-Lin Ng

Frontiers of Medicine 2015, Volume 9, Issue 3,   Pages 322-330 doi: 10.1007/s11684-015-0408-9

Abstract:

This study systematically evaluates the TCGA whole-transcriptome sequencing data of hepatocellular

Keywords： TCGA     whole-transcriptome sequencing     HCC     liver cancer    

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Highly active and durable Pd-Cu catalysts for oxygen reduction in alkaline exchange membrane fuel cells

Xiong PENG, Travis J. OMASTA, Justin M. ROLLER, William E. MUSTAIN

Frontiers in Energy 2017, Volume 11, Issue 3,   Pages 299-309 doi: 10.1007/s11708-017-0495-1

Abstract: The catalysts were physically and electrochemically characterized by X-ray diffraction (XRD), X-ray photoelectronThe Pd-Cu/Vulcan catalyst also showed higher alkaline exchange membrane fuel cell (AEMFC) performance

Keywords： alkaline exchange membrane (AEM)     fuel cell     Pd-Cu     oxygen reduction     high performance     water    

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Analysis of the genomic landscape of primary central nervous system lymphoma using whole-genome sequencing

Frontiers of Medicine   Pages 889-906 doi: 10.1007/s11684-023-0994-x

Abstract: Whole-genome sequencing was performed on 68 newly diagnosed Chinese PCNSL samples, whose genomic characteristics

Keywords： primary central nervous system lymphoma     whole-genome sequencing     TMSB4X     copy number variation    

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Whole-life Thinking and Engineering the Future

Roger Flanagan

Frontiers of Engineering Management 2014, Volume 1, Issue 3,   Pages 290-296 doi: 10.15302/J-FEM-2014040

Abstract: Whole-life thinking for engineers working on the built environment has become more important in a fastWhole-life thinking is not new, every project attempts to balance the initial capital cost with the operatingSystems thinking provides one approach to developing a more robust whole-life approach.New approaches are needed to produce more reliable whole-life predictions.Best value, rather than lowest cost, can be achieved by using whole-life appraisal as a part of the design

Keywords： whole-life thinking     systems thinking     complexity     chaos     risk management through life    

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Electrochemical CO reduction to C products over CuZn intermetallic catalysts synthesized by electrodeposition

Frontiers in Energy doi: 10.1007/s11708-023-0898-0

Abstract: was used to introduce Zn, a metal with weak *CO binding energy, into Cu to form Cu/Zn intermetallic catalystsIt was shown that, using an H-cell, the high Faradaic efficiency of C2+ hydrocarbons/alcohols

Keywords： carbon dioxide electroreduction     electrochemistry     co-electrodeposition     intermetallic catalysts     value-added    

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Oxygen reduction electrocatalysis: From conventional to single-atomic platinum-based catalysts for proton

Frontiers in Energy doi: 10.1007/s11708-023-0907-3

Abstract: Platinum (Pt)-based materials are still the most efficient and practical catalysts to drive the sluggish/nanostructuring Pt particles and modifying/innovating supports in developing conventional Pt-based catalysts, Pt single-atom catalysts (Pt SACs) as the recently burgeoning hot materials with a potential to achievesingle-atoms with carbon/non-carbon supports are emphasized in terms of the ORR activity and stability of the catalysts

Keywords： oxygen reduction electrocatalysis     Pt single-atom catalysts     conventional Pt-based catalysts     design    

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Transforming bacterial disease surveillance and investigation using whole-genome sequence to probe the

Biao Kan, Haijian Zhou, Pengcheng Du, Wen Zhang, Xin Lu, Tian Qin, Jianguo Xu

Frontiers of Medicine 2018, Volume 12, Issue 1,   Pages 23-33 doi: 10.1007/s11684-017-0607-7

Abstract:

Two decades have passed since the first bacterial whole-genome sequencing, which provides new opportunityBacterial whole-genome sequencing and bioinformatic computing allow genotyping to satisfy the requirements

Keywords： genome sequencing     genomic epidemiology     bacteria     surveillance     infectious diseases    

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Innovations of phosphorus sustainability: implications for the whole chain

Jianbo SHEN, Liyang WANG, Xiaoqiang JIAO, Fanlei MENG, Lin ZHANG, Gu FENG, Junling ZHANG, Lixing YUAN, Lin MA, Yong HOU, Tao ZHANG, Weifeng ZHANG, Guohua LI, Kai ZHANG, Fusuo ZHANG

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 4,   Pages 321-331 doi: 10.15302/J-FASE-2019283

Abstract: The innovation and feasibility of P sustainability are highlighted from the perspective of the whole

Keywords： P-use efficiency     recycling     sustainable management     the whole P chain    

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Promising approach for preparing metallic single-atom catalysts: electrochemical deposition

Frontiers in Energy 2022, Volume 16, Issue 4,   Pages 537-541 doi: 10.1007/s11708-022-0837-5

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Current challenge and perspective of PGM-free cathode catalysts for PEM fuel cells

Gang WU

Frontiers in Energy 2017, Volume 11, Issue 3,   Pages 286-298 doi: 10.1007/s11708-017-0477-3

Abstract: significantly reduce the cost of proton exchange membrane fuel cells, platinum-group metal (PGM)-free cathode catalystsCurrent M-N-C (M: Fe, Co or Mn) catalysts are considered the most promising due to their encouragingThe current Edisonian approach (i.e., trial and error) to developing PGM-free catalysts has been ineffectiveand chemical controlled precursors such as metal-organic frameworks are highly desirable for making catalysts

Keywords： oxygen reduction     fuel cells     cathode     nonprecious metal catalysts     carbon nanocomposites    

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The Research on Subject Behavioral Risk of Whole Life-cycle Water Conservation Projects

Hong-yong Liu,Ji Guo,Wei-tao He

Frontiers of Engineering Management 2014, Volume 1, Issue 4,   Pages 348-352 doi: 10.15302/J-FEM-2014048

Abstract: This article takes each participating subject in the whole life-cycle of water conservation projects

Keywords： water conservation projects     subject behavior     safety accident     risk effect     engineering ethics    

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Title Author Date Type Operation

Cell surface protein engineering for high-performance whole-cell catalysts

Hajime Nakatani,Katsutoshi Hori

Journal Article

Efficient acetoin production from pyruvate by engineered whole-cell biocatalysis

Journal Article

Efficient production of D-1,2,4-butanetriol from D-xylose by engineered

Shewei Hu, Qian Gao, Xin Wang, Jianming Yang, Nana Xu, Kequan Chen, Sheng Xu, Pingkai Ouyang

Journal Article

Organizational evolution of project management teams over the whole lifecycle of megaprojects: Case study

Journal Article

TCGA whole-transcriptome sequencing data reveals significantly dysregulated genes and signaling pathways

Daniel Wai-Hung Ho,Alan Ka-Lun Kai,Irene Oi-Lin Ng

Journal Article

Highly active and durable Pd-Cu catalysts for oxygen reduction in alkaline exchange membrane fuel cells

Xiong PENG, Travis J. OMASTA, Justin M. ROLLER, William E. MUSTAIN

Journal Article

Analysis of the genomic landscape of primary central nervous system lymphoma using whole-genome sequencing

Journal Article

Whole-life Thinking and Engineering the Future

Roger Flanagan

Journal Article

Electrochemical CO reduction to C products over CuZn intermetallic catalysts synthesized by electrodeposition

Journal Article

Oxygen reduction electrocatalysis: From conventional to single-atomic platinum-based catalysts for proton

Journal Article

Transforming bacterial disease surveillance and investigation using whole-genome sequence to probe the

Biao Kan, Haijian Zhou, Pengcheng Du, Wen Zhang, Xin Lu, Tian Qin, Jianguo Xu

Journal Article

Innovations of phosphorus sustainability: implications for the whole chain

Jianbo SHEN, Liyang WANG, Xiaoqiang JIAO, Fanlei MENG, Lin ZHANG, Gu FENG, Junling ZHANG, Lixing YUAN, Lin MA, Yong HOU, Tao ZHANG, Weifeng ZHANG, Guohua LI, Kai ZHANG, Fusuo ZHANG

Journal Article

Promising approach for preparing metallic single-atom catalysts: electrochemical deposition

Journal Article

Current challenge and perspective of PGM-free cathode catalysts for PEM fuel cells

Gang WU

Journal Article

The Research on Subject Behavioral Risk of Whole Life-cycle Water Conservation Projects

Hong-yong Liu,Ji Guo,Wei-tao He

Journal Article